The CNAG Biorepository collects, quantifies and stores the DNA and RNA samples for sequencing requests.
We work closely with our collaborators to ensure that appropriate DNA/RNA extraction procedures are followed, to determine the sample requirements for the experiments to be conducted by the CNAG and to coordinate the sample shipment. Barcode labels and tubes are sent to the collaborators for safe tracking of samples.
At the CNAG, samples are checked for purity and integrity before processing. DNA integrity is assessed on agarose gel. The DNA QC includes detection of incidental present inhibitors by regular PCR amplification and spectrophotometric measurements. Concentration is verified by fluorometry (Picogreen assay). RNA is subjected to rigorous quality control to monitor degradation on the Agilent 2100 Bioanalyzer and quantitated on the Nanodrop Spectrophotometer.
Samples are stored under highly controlled conditions and, if appropriate, distributed in several aliquots to avoid thawing and refreezing.